Multi-scale 3D virtual histology via phase-contrast X-ray tomography with synchrotron radiation

Frohn, Jasper

dc.contributor.author	Frohn, Jasper
dc.date.accessioned	2023-11-16T10:16:02Z
dc.date.available	2023-11-16T10:16:02Z
dc.date.issued	2023	de
dc.identifier.uri	https://doi.org/10.17875/gup2023-2409
dc.format.extent	ii, 136	de
dc.format.medium	Print	de
dc.language.iso	eng	de
dc.relation.ispartofseries	Göttingen Series in X-ray Physics	de
dc.rights.uri	https://creativecommons.org/licenses/by-sa/4.0/deed.de
dc.subject.ddc	530	de
dc.subject.other	OAPEN	de
dc.title	Multi-scale 3D virtual histology via phase-contrast X-ray tomography with synchrotron radiation	de
dc.type	monograph	de
dc.price.print	47,00
dc.identifier.urn	urn:nbn:de:gbv:7-isbn-978-3-86395-601-1-0
dc.description.print	Softcover, 17x24	de
dc.subject.division	peerReviewed	de
dc.subject.subjectheading	Physik	de
dc.relation.isbn-13	978-3-86395-601-1
dc.identifier.articlenumber	8102325	de
dc.identifier.intern	isbn-978-3-86395-601-1	de
dc.bibliographicCitation.volume	030	de
dc.type.subtype	thesis	de
dc.subject.bisac	SCI055000	de
dc.subject.vlb	640	de
dc.subject.bic	PH	de
dc.description.abstracteng	To this day, the standard method for investigating biological tissue with cellular resolution is the examination under a light microscope, first denoted as histology by Karl Meyer in 1819. Despite the enormous success and importance of histology, it has two major disadvantages. Firstly, the specimen must be physically cut into thin sections due to the limited penetrating power of optical light, and secondly, additional staining of the specimen is required to achieve sufficient image contrast. Both disadvantages can be overcome by the non-destructive method of propagation-based X-ray phase-contrast tomography. While the mechanism of phase-contrast provides sufficient image contrast to image single cells, a tomographic imaging scheme with penetrating X-rays allows for an undamaged sample by virtually slicing the reconstructed 3D sample volume. In this work, the holotomography setup of the synchrotron endstation „GINIX“ (The Göttingen Instrument for Nanoscale-Imaging with X-Rays) was extended to a multi-scale X-ray phase-contrast tomography setup suitable for 3D virtual histology by adding two acquisition schemes. Compared to the existing setup, the first additional scheme is a propagation-based microtomography setup, which enlarges the reconstructed 3D volumes by a factor of approx. 64 at a fraction of the acquisition time (ca. 2 min). The second additional scheme aims for higher resolutions. To this end, the X-ray waveguide illumination was combined with photon counting detector with a large field of view and a novel phase reconstruction scheme, which is based on iterative farfield phase retrieval without an „empty-beam correction“ in the detector plane.	de
dc.subject.eng	biological tissue	de
dc.subject.eng	3D virtual histology	de
dc.subject.eng	multiscale approach	de
dc.notes.vlb-print	lieferbar
dc.intern.doi	10.17875/gup2023-2409	de
dc.identifier.purl	http://resolver.sub.uni-goettingen.de/purl?univerlag-isbn-978-3-86395-601-1
dc.format.chapters	-	de
dc.intern.asin	386395601x

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